Sequence of the neuraminidase gene of an avian influenza a virus (A/parrot/Ulster/73, H7N1)
Identifieur interne : 002500 ( Main/Exploration ); précédent : 002499; suivant : 002501Sequence of the neuraminidase gene of an avian influenza a virus (A/parrot/Ulster/73, H7N1)
Auteurs : Harry Steuler [Allemagne] ; Wolfgang Rohde [Allemagne] ; Christoph Scholtissek [Allemagne]Source :
- Virology [ 0042-6822 ] ; 1984.
English descriptors
- Teeft :
- Amino, Amino acid sequence, Amino acids, Complete nucleotide sequence, Complete sequence, Cysteine, Deletion, Gene sequencing, Glycosylation, Head region, Influenza, Influenza virus, Molecular weight, Neuraminidase, Neuraminidase gene, Neuraminidase genes, Neuraminidases, Parrot, Parrot ulster, Parrot ulster strain, Potential glycosylation sites, Sequencing, Udorn, Ulster.
Abstract
Abstract: The complete sequence of the neuraminidase (NA) gene of the influenza A strain A/parrot/Ulster/73 (Fi7N1) has been determined after reverse transcribing and cloning it into the pBR322 plasmid, followed by subcloning into M13 vectors and sequencing with dideoxynucleotide chain terminators. The gene consists of 1458 nucleotides and codes for a protein of 469 amino acids. The neuraminidase has seven potential glycosylation sites. According to the molecular weight as determined by electrophoretic migration in polyacrylamide gel all of these sites might carry a carbohydrate side chain. When the parrot Ulster NA was compared with two other N1 neuraminidases, those of the human PR8 and WSN strains, deletions in the stalk region of 15 amino acids for PR8 NA and of 16 amino acids for WSN NA were apparent. No further rearrangements were found within N1 neuraminidases. Although the parrot Ulster strain was isolated 40 years after the two human strains, the base sequence homology of their NA genes is still 83 or 82%, respectively.
Url:
DOI: 10.1016/0042-6822(84)90122-3
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000B48
- to stream Istex, to step Curation: 000B48
- to stream Istex, to step Checkpoint: 001170
- to stream Main, to step Merge: 002626
- to stream Main, to step Curation: 002500
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Sequence of the neuraminidase gene of an avian influenza a virus (A/parrot/Ulster/73, H7N1)</title><author><name sortKey="Steuler, Harry" sort="Steuler, Harry" uniqKey="Steuler H" first="Harry" last="Steuler">Harry Steuler</name></author><author><name sortKey="Rohde, Wolfgang" sort="Rohde, Wolfgang" uniqKey="Rohde W" first="Wolfgang" last="Rohde">Wolfgang Rohde</name></author><author><name sortKey="Scholtissek, Christoph" sort="Scholtissek, Christoph" uniqKey="Scholtissek C" first="Christoph" last="Scholtissek">Christoph Scholtissek</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:8641C5FDEFADA571514ABAA8188CE615B230F345</idno><date when="1984" year="1984">1984</date><idno type="doi">10.1016/0042-6822(84)90122-3</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-6W27Z7ZV-K/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000B48</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000B48</idno><idno type="wicri:Area/Istex/Curation">000B48</idno><idno type="wicri:Area/Istex/Checkpoint">001170</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001170</idno><idno type="wicri:doubleKey">0042-6822:1984:Steuler H:sequence:of:the</idno><idno type="wicri:Area/Main/Merge">002626</idno><idno type="wicri:Area/Main/Curation">002500</idno><idno type="wicri:Area/Main/Exploration">002500</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Sequence of the neuraminidase gene of an avian influenza a virus (A/parrot/Ulster/73, H7N1)</title><author><name sortKey="Steuler, Harry" sort="Steuler, Harry" uniqKey="Steuler H" first="Harry" last="Steuler">Harry Steuler</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>In stitut für Virologtie, Justus-I,ielng-Universität Giessen, 6800 Giessen</wicri:regionArea><wicri:noRegion>6800 Giessen</wicri:noRegion><wicri:noRegion>6800 Giessen</wicri:noRegion></affiliation></author><author><name sortKey="Rohde, Wolfgang" sort="Rohde, Wolfgang" uniqKey="Rohde W" first="Wolfgang" last="Rohde">Wolfgang Rohde</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>In stitut für Virologtie, Justus-I,ielng-Universität Giessen, 6800 Giessen</wicri:regionArea><wicri:noRegion>6800 Giessen</wicri:noRegion><wicri:noRegion>6800 Giessen</wicri:noRegion></affiliation></author><author><name sortKey="Scholtissek, Christoph" sort="Scholtissek, Christoph" uniqKey="Scholtissek C" first="Christoph" last="Scholtissek">Christoph Scholtissek</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>In stitut für Virologtie, Justus-I,ielng-Universität Giessen, 6800 Giessen</wicri:regionArea><wicri:noRegion>6800 Giessen</wicri:noRegion><wicri:noRegion>6800 Giessen</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1984">1984</date><biblScope unit="volume">135</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="118">118</biblScope><biblScope unit="page" to="124">124</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amino</term><term>Amino acid sequence</term><term>Amino acids</term><term>Complete nucleotide sequence</term><term>Complete sequence</term><term>Cysteine</term><term>Deletion</term><term>Gene sequencing</term><term>Glycosylation</term><term>Head region</term><term>Influenza</term><term>Influenza virus</term><term>Molecular weight</term><term>Neuraminidase</term><term>Neuraminidase gene</term><term>Neuraminidase genes</term><term>Neuraminidases</term><term>Parrot</term><term>Parrot ulster</term><term>Parrot ulster strain</term><term>Potential glycosylation sites</term><term>Sequencing</term><term>Udorn</term><term>Ulster</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The complete sequence of the neuraminidase (NA) gene of the influenza A strain A/parrot/Ulster/73 (Fi7N1) has been determined after reverse transcribing and cloning it into the pBR322 plasmid, followed by subcloning into M13 vectors and sequencing with dideoxynucleotide chain terminators. The gene consists of 1458 nucleotides and codes for a protein of 469 amino acids. The neuraminidase has seven potential glycosylation sites. According to the molecular weight as determined by electrophoretic migration in polyacrylamide gel all of these sites might carry a carbohydrate side chain. When the parrot Ulster NA was compared with two other N1 neuraminidases, those of the human PR8 and WSN strains, deletions in the stalk region of 15 amino acids for PR8 NA and of 16 amino acids for WSN NA were apparent. No further rearrangements were found within N1 neuraminidases. Although the parrot Ulster strain was isolated 40 years after the two human strains, the base sequence homology of their NA genes is still 83 or 82%, respectively.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country></list><tree><country name="Allemagne"><noRegion><name sortKey="Steuler, Harry" sort="Steuler, Harry" uniqKey="Steuler H" first="Harry" last="Steuler">Harry Steuler</name></noRegion><name sortKey="Rohde, Wolfgang" sort="Rohde, Wolfgang" uniqKey="Rohde W" first="Wolfgang" last="Rohde">Wolfgang Rohde</name><name sortKey="Scholtissek, Christoph" sort="Scholtissek, Christoph" uniqKey="Scholtissek C" first="Christoph" last="Scholtissek">Christoph Scholtissek</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002500 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002500 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:8641C5FDEFADA571514ABAA8188CE615B230F345
|texte= Sequence of the neuraminidase gene of an avian influenza a virus (A/parrot/Ulster/73, H7N1)
}}
| This area was generated with Dilib version V0.6.33. |  |